Publications

*equal contribution ^#co-corresponding author Shao lab members

The eRF1 degrader SRI-41315 acts as a molecular glue at the ribosomal decoding center.
Coelho JPL*, Yip MCJ*, Oltion K, Taunton J, and Shao S. (2024) Nat. Chem. Biol. doi: 10.1038/s41589-023-01521-0. PMID 38172604.

The endoplasmic reticulum and the fidelity of nascent protein localization.
McKenna MJ and Shao S. (2023) Cold Spring Harb. Perspect. Biol. 15:a041249. PMID 36041782.

Protein biosynthesis at the ER: finding the right accessories.
Shao S. (2023) Mol. Biol. Cell 34:pe1. doi: 10.1019/mbc.E21-09-0451. PMID 36520029.

ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.
McKenna MJ, Adams BM, Chu V, Paulo JA, and Shao S. (2022) Mol. Cell 82:4277-4289.e10. PMID 36283413.

Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes.
Juette MF*, Carelli JD*, Rundlet EJ*, Brown A, Shao S, Ferguson A, Wasserman MR, Holm M, Taunton J^#, and Blanchard SC^#. (2022) eLife 11:e81608. PMID 36264623.

Mechanism of client selection by the protein quality control factor UBE2O.
Yip MCJ*, Sedor SF*, and Shao S. (2022) Nat. Struct. Mol. Biol. 29:774-780. PMID 35915257.

Cryo-EM structure of an active bacterial TIR-STING filament complex.
Morehouse BR, Yip MCJ, Keszei AFA, McNamara-Bordewick NK, Shao S^#, and Kranzusch PJ^#. (2022) Nature 608:803-807. PMID 35869168.

A 'NAC' for targeting proteins to the ER.
Coelho JPL and Shao S. (2022) Trends Biochem. Sci. 47:730-731. PMID 35501234.

Structural insights into metazoan pretargeting GET complexes.
Keszei AFA, Yip MCJ, Hsieh TC, and Shao S. (2021) Nat. Struct. Mol. Biol. 28:1029-1037. PMID 34887561.

Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.
Tal N*, Morehouse BR*, Millman A, Stokar-Avihail A, Avraham C, Fedorenko T, Yirmiya E, Herbst E, Brandis A, Mehlman T, Oppenheimer-Shaanan Y, Keszei AFA, Shao S, Amitai G, Kranzusch PJ^#, and Sorek R^#. (2021) Cell 184:5728-5739.e16. PMID 34644530.

Selective destabilization of polypeptides synthesized from NMD-targeted transcripts.
Chu V, Feng Q, Lim Y, and Shao S. (2021) Mol. Biol. Cell 32:ar38. PMID 34586879.

Discovery of C13-aminobenzoyl cycloheximide derivatives that potently inhibit translation elongation.
Koga Y, Hoang EM, Park Y, Keszei AFA, Murray J, Shao S^#, and Liau BB^#. (2021) J Am. Chem. Soc. 143:13473-13477. PMID 34403584.

Detecting and rescuing stalled ribosomes.
Yip MCJ and Shao S. (2021) Trends Biochem. Sci. 46:731-743. PMID 33966939.

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
McKenna MJ*, Sim SI*, Ordureau A, Wei L, Harper JW, Shao S^#, and Park E^#. (2020) Science 369:eabc5809. PMID 32973005.

STING cyclic dinucleotide sensing originated in bacteria.
Morehouse BR, Govande AA, Millman A, Keszei AFA, Lowey B, Ofir G, Shao S, Sorek R, and Kranzusch PJ. (2020) Nature 586:429-433. PMID 32877915.

CBASS immunity uses CARF-related effectors to sense 3'-5'- and 2'-5'-linked cyclic oligonucleotide signals and protect bacteria from phage infection.
Lowey B, Whiteley AT, Keszei AFA, Morehouse BR, Mathews IT, Antine SP, Cabrera VJ, Kashin D, Niemann P, Jain M, Schwede F, Mekalanos JJ, Shao S, Lee ASY, and Kranzusch PJ. (2020) Cell 182:38-49. PMID 32544385.

ELAC1 repairs tRNAs cleaved during ribosome-associated quality control.
Yip MCJ, Savickas S, Gygi SP, and Shao S. (2020) Cell Rep. 30:2106-2114. PMID 32075755.

TTC5 mediates autoregulation of tubulin via mRNA degradation.
Lin Z, Gasic I, Chandrasekaran V, Peters N, Shao S, Mitchison TJ, and Hegde RS. (2020) Science 367:100-104. PMID 31727855.

Mechanism of ribosome stalling during translation of a poly(A) tail.
Chandrasekaran V*, Juszkiewicz S*, Choi J, Puglisi JD, Brown A, Shao S, Ramakrishnan V, and Hegde RS. (2019) Nat. Struct. Mol. Biol. 26:1132-1140. PMID 31768042.

Mechanism for recycling tRNAs on stalled ribosomes.
Yip MCJ, Keszei AFA, Feng Q, Chu V, McKenna MJ, and Shao S. (2019) Nat. Struct. Mol. Biol. 26:343-349. PMID 31011209.

Activation of GCN2 by the ribosomal P-stalk.
Inglis AJ, Masson GR, Shao S, Perisic O, McLaughlin SH, Hegde RS, and Williams RL. (2019) Proc. Natl. Acad. Sci. 116:4946-4954. PMID 30804176.

EMC is required to initiate accurate membrane protein topogenesis.
Chitwood PJ, Juszkiewicz S, Guna A, Shao S, and Hegde RS. (2018) Cell 175:1507-1519. PMID 30415835.

Structures of translationally inactive mammalian ribosomes.
Brown A^#, Baird MR, Yip MCJ, Murray J, and Shao S^#. (2018) eLife 7:e40486. PMID 30355441.

Ribosomes and cryo-EM: a duet.
Brown A^# and Shao S^#. (2018) Curr. Opin. Struct. Biol. 52:1-7 (review). PMID 30015201.

In vitro reconstitution of translational arrest pathways.
Feng Q and Shao S. (2018) Methods 137:20-36. PMID 29277545.

Mechanistic basis for a molecular triage reaction.
Shao S, Rodrigo-Brenni MC, Kivlen MH, and Hegde RS. (2017) Science 355:298-302. PMID 28104892.

Decoding mammalian ribosome-mRNA states by translational GTPase complexes.
Shao S*, Murray J*, Brown A*, Taunton J, Ramakrishnan V, and Hegde RS. (2016) Cell 167:1229-1249. PMID 27863242.

Ubiquilins chaperone and triage mitochondrial membrane proteins for degradation.
Itakura E, Zavodszky E, Shao S, Wohlever ML, Keenan RJ, and Hegde RS. (2016) Mol. Cell 63:21-33. PMID 27345149.

Target selection during protein quality control.
Shao S^#and Hegde RS^#. (2016) Trends Biochem. Sci. 41:124-137 (review). PMID 26628391.

Structural basis for stop codon recognition in eukaryotes.
Brown A*, Shao S*, Murray J, Hegde RS, and Ramakrishnan V. (2015) Nature 524:493-496. PMID 26245381.

The ribosome quality control pathway can access nascent polypeptides stalled at the Sec61 translocon.
Von der Malsburg K, Shao S, and Hegde RS. (2015) Mol. Biol. Cell 26:2168-2180. PMID 25877867.

Structure and assembly pathway of the ribosome quality control complex.
Shao S, Brown A, Santhanam B, and Hegde RS. (2015) Mol. Cell 57:433-444. PMID 25578875.

Local synthesis and disposal.
Shao S and Hegde RS. (2014) Science 346:701-702 (preview). PMID 25378608.

Reconstitution of a minimal ribosome-associated ubiquitination pathway with purified factors.
Shao S and Hegde RS. (2014) Mol. Cell 55:880-890. PMID 25132172.

Listerin-dependent nascent protein ubiquitination relies on ribosome subunit dissociation.
Shao S*, von der Malsburg K*, and Hegde RS. (2013) Mol. Cell 50:637-648. PMID 23685075.

The budding yeast nuclear envelope adjacent to the nucleolus serves as a membrane sink during mitotic delay.
Witkin KL, Chong Y, Shao S, Webster MT, Lahiri S, Walters AD, Lee B, Koh JL, Prinz WA, Andrews BJ, and Cohen-Fix O. (2012) Curr. Biol. 22:1128-1133. PMID 22658600.

A calmodulin-dependent translocation pathway for small secretory proteins.
Shao S and Hegde RS. (2011) Cell 147:1576-1588. PMID 22196732.

Membrane protein insertion at the endoplasmic reticulum.
Shao S and Hegde RS. (2011) Annu. Rev. Cell Dev. Biol. 27:25-56 (review). PMID 21801011.

A flip turn for membrane protein insertion.
Shao S and Hegde RS. (2011) Cell 146:13-15 (preview). PMID 21729778.

Tracking F plasmid TraI relaxase processing reactions provides insight into F plasmid transfer.
Dostál L, Shao S, and Schildbach JF. (2011) Nucleic Acids Res. 39:2658-2670. PMID 21109533.